A Microwell Device for the Efficient Generation of Arrays of Microtissues and Humanized Bone Marrow Micro-Ossicles

(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method the assembly of arrays microtissues from stromal cells (BMSC) in vitro and their maturation into micro-ossicles vivo. (2) Methods: Discs with 50 microwells were used to assemble 3 × 105 BMSCs each on nylon mesh carrier. Microtissues cultured chondrogenic induction medium followed by hypertrophic an attempt drive endochondral ossification, then they implanted NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, where remodeled Mice transplanted human umbilical cord blood CD34+ cells. (3) Results: Micro-ossicles contained more CD45+ cells, but fewer progenitor than mouse marrow. Human hematopoietic cycle rapidly at non-physiological rates marrow, reduced cell content is consistent notion that humanized niche better controls cycling. (4) Conclusions: Assembling microwells, linked membrane carrier, provides elegant manufacture handle organ-like properties. More generally, this approach platform could aid bridging gap between manipulation vivo implantation.